Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Cell-Free Scaled Production and Adjuvant Addition …
JoVE Journal
Immunology and Infection

Ce contenu est disponible gratuitement.

JoVE Journal Immunology and Infection
Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development
 

Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development

Article DOI: 10.3791/63028-v • 12:53 min • March 16th, 2022
March 16th, 2022 •
*1, *1, 1, 2, 2, 1, 1, 1, 1, 1, 2, 1,3
1Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, 2Department of Pathology and Laboratory Medicine, University of California, 3School of Medicine, Radiation Oncology, University of California Davis
* These authors contributed equally

Chapitres

résumé
Automatic Translation

This protocol describes using commercial, cell-free protein expression kits to produce membrane proteins supported in nanodisc that can be used as antigens in subunit vaccines.

Tags

Cell-Free Scaled Production Adjuvant Addition Recombinant Major Outer Membrane Protein Chlamydia Muridarum Vaccine Development Subunit Vaccine Antigens Membrane Proteins Protein Production Protein Purification Lipid Membrane Native-like Confirmation Formulation With Adjuvants Cell-free Expression Tagless Structure Scalable Process Commercial Companies Chlamydia Antigen Pathogen Vaccine Development Cancer Vaccine Development MOMP-tNLPs Reconstitution Buffer EDTA-free Protease Inhibitor
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter