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Biochemistry

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JoVE Journal Biochemistry
Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
 

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B

Article DOI: 10.3791/63118-v • 11:44 min • January 19th, 2022
January 19th, 2022 •
1,2, 1, 1, 1, 1
1Department of Biological Sciences, The University of Alabama, 2Department of Pharmaceutical Botany, Faculty of Pharmacy, Hacettepe University

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résumé
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The protocol describes the preparation of cell membrane affinity chromatography (CMAC) columns with immobilized cell membrane fragments containing functional transmembrane tropomyosin kinase receptor B proteins. The use of CMAC columns in the identification of specialized plant metabolites interacting with these receptors and present in complex natural mixtures is also explained.

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Keywords: Cellular Membrane Affinity Chromatography Transmembrane Receptors Plant Metabolites Tropomyosin Kinase Receptor Benzamidine PMSF Protease Inhibitor ATP Tris-HCl Buffer Homogenization Buffer Cell Harvesting Cell Lysis Column Preparation
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