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JoVE Journal Neuroscience
Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies
 

Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies

Article DOI: 10.3791/63126-v • 12:28 min • March 12th, 2022
March 12th, 2022 •
1,2, 1,2,4, 1,2, 3, 1,2, 1,2
1Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, 2Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), 3Vitreo-retinal Department, Centro Privado de Ojos Romagosa-Fundación VER, 4Unidad de Investigación y Desarrollo en Tecnología Farmacéutica (UNITEFA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)

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This article describes a well-established and reproducible lectin stain assay for the whole mount retinal preparations and the protocols required for the quantitative measurement of vascular parameters frequently altered in proliferative and non-proliferative retinopathies.

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Keywords: Vascular Parameters Whole Mount Retinas Non-proliferative Retinopathy Proliferative Retinopathy Oxygen-induced Retinopathy Metabolic Syndrome Vascular Alterations Avascular Areas Neovascular Areas Arteriole Dilatation Arteriole Tortuosity Vascular Density Vascular Branching Retinal Dissection Immunohistochemistry Isolectin Staining
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