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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualization of Inflammatory Caspases Induced Proximity in Human Monocyte-Derived Macrophages
 

Visualization of Inflammatory Caspases Induced Proximity in Human Monocyte-Derived Macrophages

Article DOI: 10.3791/63162-v • 08:41 min • April 6th, 2022
April 6th, 2022 •
1, 1
1Department of Pediatrics, Division of Hematology-Oncology, Department of Molecular and Cellular Biology, Texas Children’s Hospital William T. Shearer Center for Human Immunobiology, Baylor College of Medicine

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This protocol describes the workflow to obtain monocytes-derived macrophages (MDM) from human blood samples, a simple method to efficiently introduce inflammatory caspase Bimolecular Fluorescence Complementation (BiFC) reporters into human MDM without compromising cell viability and behavior, and an imaging-based approach to measure inflammatory caspase activation in living cells.

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Visualization Inflammatory Caspases Induced Proximity Human Monocyte-derived Macrophages Molecular Level Living Cells Disease State Technique Components Requirements Localization Activation Complexes Primary Immune Cells Optimizations Adapted Range Of Proteins Oligomerization Applicability Microscopic Methodologies Protocol
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