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JoVE Journal Bioengineering
Simplified, High-throughput Analysis of Single-cell Contractility using Micropatterned Elastomers
 

Simplified, High-throughput Analysis of Single-cell Contractility using Micropatterned Elastomers

Article DOI: 10.3791/63211-v • 14:33 min • April 8th, 2022
April 8th, 2022 •
1, 1, 1, 1, 1, 1,2, 1
1Forcyte Biotechnologies, 2University of California, Los Angeles

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This work presents a flexible protocol for utilizing fluorescently labeled elastomeric contractible surfaces (FLECS) Technology in microwell format for simplified, hands-off quantification of single-cell contractile forces based on visualized displacements of fluorescent protein micropatterns.

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Keywords: Single-cell Contractility Micropatterned Elastomers Cell-generated Mechanical Forces Organ Function Smooth Muscle Cell Contraction Contractile Mechanisms Cell Contractile Function Drug Screening Microtechnology Fluorescently Labeled Elastomeric Contractable Surfaces Protein Micropatterning Cell Position And Shape Cell Traction Forces Image Analysis Cellular Force Biology
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