Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Medicine / Low-input Nucleus Isolation and Multiplexing with …
JoVE Journal
Medicine

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

JoVE Journal Medicine
Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing
 

Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing

Article DOI: 10.3791/63397-v • 10:44 min • March 23rd, 2022
March 23rd, 2022 •
1,2, 1, 1,2, 1, 2, 1, 3, 1,2
1Department of Anatomy & Embryology, Leiden University Medical Center, 2Department of Cardiology, Leiden University Medical Center, 3Department of Medical Statistics and Bioinformatics, Leiden University Medical Center

Chapitres

résumé
Automatic Translation

This protocol describes the detailed, low-input sample preparation for single-nucleus sequencing, including the dissection of mouse superior cervical and stellate ganglia, cell dissociation, cryopreservation, nucleus isolation, and hashtag barcoding.

Tags

Keywords: Single-nucleus RNA Sequencing Cardiac Sympathetic Neurons Droplet-based Sequencing Hashtag Oligos Multiplexing Superior Cervical Ganglia Stellate Ganglia Mouse Tissue Dissection Trypsin Digestion
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter