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Bioengineering
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JoVE Journal Bioengineering
Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
 

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Article DOI: 10.3791/63523-v • 14:12 min • November 21st, 2023
November 21st, 2023 •
1, 1, 1, 2, 1, 1, 1,2
1Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, 2Graduate School of Engineering, Osaka University

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This protocol describes how to build a continuous-flow-polymerase chain system based on a microfluidic chip and how to build a capillary electrophoresis system in the lab. It presents a simple method for the analysis of nucleic acids in the lab.

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Keywords: Continuous-flow PCR Microfluidic Chip Capillary Electrophoresis Nucleic Acid Amplification Escherichia Coli Photolithography SU-8 Photoresist Soft Bake Exposure Post-exposure Bake
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