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JoVE Journal Biochemistry
Purification of Ubiquitinated p53 Proteins from Mammalian Cells
 

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Article DOI: 10.3791/63602-v • 10:55 min • March 21st, 2022
March 21st, 2022 •
1, 1, 1
1Center for Translational Medicine, The Affiliated Zhangjiagang Hospital of Soochow University

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résumé
Automatic Translation

The protocol describes a step-by-step method to purify ubiquitinated proteins from mammalian cells using the p53 tumor suppressor protein as an example. Ubiquitinated p53 proteins were purified from cells under stringent nondenaturing and denaturing conditions.

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Keywords: Ubiquitinated P53 Protein Purification Mammalian Cells Ubiquitination Liposome Transfection MG132 FLAG Lysis Buffer Protease Inhibitor
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