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Biochemistry

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JoVE Journal Biochemistry
Avance de imágenes de alta resolución de ensamblajes de virus en líquido y hielo
 
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Avance de imágenes de alta resolución de ensamblajes de virus en líquido y hielo

Article DOI: 10.3791/63856-v • 08:31 min • July 20th, 2022
July 20th, 2022 •
*1,2, *1, *1,3, 1,4, 1,4, 5, 6, 1, 7, 8, 2, 9, 1,5,6
1Department of Biomedical Engineering, Pennsylvania State University, 2Department of Materials Science and Engineering, McMaster University, 3Bioinformatics and Genomics Graduate Program, Huck Institutes of the Life Sciences, Pennsylvania State University, 4Molecular, Cellular, and Integrative Biosciences Graduate Program, Huck Institutes of the Life Sciences, Pennsylvania State University, 5Materials Research Institute, Pennsylvania State University, 6Huck Institutes of the Life Sciences, Pennsylvania State University, 7Applications team, Direct Electron, 8Application Scientist, Protochips, Inc., 9Department of Biology, Wake Forest University
* These authors contributed equally

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Aquí se describen los protocolos para preparar conjuntos de virus adecuados para el análisis de EM líquido y crio-EM a nanoescala utilizando microscopía electrónica de transmisión.

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Bioquímica Número 185 microscopía electrónica de transmisión TEM EM líquido crio-EM virus adenoasociados SARS-CoV-2 partículas de doble capa de rotavirus DLP biología estructural
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