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Neuroscience

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Immunolabeling and Counting Ribbon Synapses in Young Adult and Aged Gerbil Cochleae
 

Immunolabeling and Counting Ribbon Synapses in Young Adult and Aged Gerbil Cochleae

Article DOI: 10.3791/63874-v 08:25 min April 21st, 2022
April 21st, 2022

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résumé

A protocol for processing young adult and aged gerbil cochleae by immunolabeling the afferent synaptic structures and hair cells, quenching autofluorescence in aged tissue, dissecting and estimating the length of the cochleae, and quantifying the synapses in image stacks obtained with confocal imaging is presented.

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Keywords: Immunolabeling Ribbon Synapses Gerbil Cochlea Aging Synaptopathy Cochlear Dissection Autofluorescence Quencher Cochlear Half Razor Blade Spring Scissors Forceps
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