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Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues
 

Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues

Article DOI: 10.3791/63990-v • 09:57 min • October 17th, 2022
October 17th, 2022 •
*1,2, *1,3, 1,2, 1,4, 1,5, 1, 2, 1,6, 1
1State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, 2Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, 3Department of VIP Dental Care, School of Stomatology, The Fourth Military Medical University, 4School of Basic Medicine, The Fourth Military Medical University, 5College of Life Science, Northwest University, 6Xi'an Institute of Tissue Engineering and Regenerative Medicine
* These authors contributed equally

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résumé
Automatic Translation

The present protocol describes a method to extract extracellular vesicles from the peripheral blood and solid tissues with subsequent profiling of surface antigens and protein cargos.

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Keywords: Extracellular Vesicles Isolation Analysis Plasma Solid Tissues Surface Antigens Protein Cargoes Flow Cytometry Maxillary Bone Liberase Centrifugation PBS Tissue Preparation Sample Processing
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