Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Enrichment of Extracellular Matrix Proteins from…
JoVE Journal
Biology

Ce contenu est en libre accès.

JoVE Journal Biology
Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis
 

Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis

Article DOI: 10.3791/53057-v • 07:28 min • July 23rd, 2015
July 23rd, 2015 •
1, 2, 1
1Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, 2Proteomics Platform, Broad Institute

Chapitres

résumé
Automatic Translation

This protocol describes a procedure for enriching ECM proteins from tissues or tumors and deglycosylating and digesting the ECM-enriched preparations into peptides to analyze their protein composition by mass spectrometry.

Tags

Extracellular Matrix Proteins Digestion Peptides Mass Spectrometry Analysis Tissue Enrichment Decellularization Procedure ECM Composition Biomarkers Therapeutic Targets Biochemical Analysis Insolubility Of ECM Proteins Deglycosylation Protein Preparation Mass Spectrometry Analysis
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter