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Developmental Biology

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JoVE Journal Developmental Biology
A Guide to Examining Intramuscular Fat Formation and its Cellular Origin in Skeletal Muscle
 

A Guide to Examining Intramuscular Fat Formation and its Cellular Origin in Skeletal Muscle

Article DOI: 10.3791/63996-v • 09:19 min • May 26th, 2022
May 26th, 2022 •
1, 1, 1,2
1Department of Pharmacology and Therapeutics, University of Florida College of Medicine, 2Myology Institute, University of Florida College of Medicine

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The replacement of healthy muscle tissue with intramuscular fat is a prominent feature of human diseases and conditions. This protocol outlines how to visualize, image, and quantify intramuscular fat, allowing the rigorous study of the mechanisms underlying intramuscular fat formation.

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Keywords: Intramuscular Fat Sarcopenia Neuromuscular Diseases Adipocyte Morphology Skeletal Muscle Duchenne Muscular Dystrophy Glycerol Tibialis Anterior Tissue Preservation Histology
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