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Large-Animal Model of Donation after Circulatory Death and Normothermic Regional Perfusion for Cardiac Assessment
 

Large-Animal Model of Donation after Circulatory Death and Normothermic Regional Perfusion for Cardiac Assessment

Article DOI: 10.3791/64009-v • 07:49 min • May 10th, 2022
May 10th, 2022 •
*1,2, *3, 4,5, 4,5, 1,2, 1,2
1Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), 2Department of Surgery, Faculty of Medicine, Université de Montréal, 3Division of Cardiac Surgery, Dalhousie University, 4Division of Cardiovascular Surgery, Peter Munk Cardiac Centre, Toronto General Hospital, 5Department of Surgery, University of Toronto
* These authors contributed equally

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The protocol describes a large-animal (porcine) model of donation after circulatory death, followed by thoracoabdominal normothermic regional perfusion that closely simulates the clinical scenario in heart transplantation, and has the potential to facilitate therapeutic studies and strategies.

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Keywords: Large-animal Model Donation After Circulatory Death Normothermic Regional Perfusion Cardiac Assessment Translational Research Boar Swine Heart Transplantation Midline Incision Sternotomy Pericardiotomy Heparin Anticoagulation Activated Clotting Time Propofol Circulatory Arrest Functional Warm Ischemia Time Aortic Cross-clamping Metzenbaum Scissors
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