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JoVE Journal Developmental Biology
Anvendelse af bisection til at reducere mitokondrie-DNA i bovine oocyte
 
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Anvendelse af bisection til at reducere mitokondrie-DNA i bovine oocyte

Article DOI: 10.3791/64060-v • 06:15 min • July 6th, 2022
July 6th, 2022 •
*1, *1, 2, 2, 2, 3, 1
1Department of Animal, Dairy, and Veterinary Sciences, Utah State University, 2Reproductive Sciences, Beckman Center for Conservation Research, San Diego Zoo Wildlife Alliance, 3Genus PLC
* These authors contributed equally

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Her præsenterer vi en protokol for signifikant at reducere mitokondrie-DNA-kopinumrene i en bovin oocyt (P < 0,0001). Denne metode anvender centrifugering og bisektion til væsentligt at reducere oocyt mitokondrier og kan give mulighed for en øget chance for udvikling i de rekonstruerede interspecies somatiske celle nukleare overførselsembryoner.

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Udviklingsbiologi udgave 185
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