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Immunology and Infection

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JoVE Journal Immunology and Infection
Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis
 

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis

Article DOI: 10.3791/64212-v • 08:44 min • September 7th, 2022
September 7th, 2022 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2
1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, 2Research Unit of Critical Infection in Children, 2019RU016, Chinese Academy of Medical Sciences

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We describe a method combining immunomagnetic beads and fluorescence-activated cell sorting to isolate and analyze defined immune cell subpopulations of peripheral blood mononuclear cells (monocytes, CD4+ T cells, CD8+ T cells, B cells, and natural killer cells). Using this method, magnetic and fluorescently labeled cells can be purified and analyzed.

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Immune Cell Subpopulations Peripheral Blood Samples Children Infectious Mononucleosis Characterization EBV Infection Linlin Zhang PBMCs Microcentrifuge Tube Spin Supernatant Buffer CD 14 Microbeads Incubate Wash Centrifuge Magnetic Separation Column Magnetic Bead Separator Cell Suspension Unlabeled Cells Effluent
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