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JoVE Journal Neuroscience
Quantification of Immunostained Caspase-9 in Retinal Tissue
 

Quantification of Immunostained Caspase-9 in Retinal Tissue

Article DOI: 10.3791/64237-v • 05:18 min • July 25th, 2022
July 25th, 2022 •
1, 2, 1, 1, 3, 1, *1, *1,4,5
1Department of Pathology & Cell Biology, Vagelos College of Physicians and Surgeons, Columbia University, 2Department of Molecular Pharmacology and Therapeutics, Vagelos College of Physicians and Surgeons, Columbia University, 3NCI-designated Cancer Center, Sanford Burnham Prebys Medical Discovery Institute, 4Department of Neurology, Vagelos College of Physicians and Surgeons, Columbia University, 5The Taub Institute for Research on Alzheimer’s Disease and the Aging Brain, Vagelos College of Physicians and Surgeons, Columbia University
* These authors contributed equally

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Presented here is a detailed immunohistochemistry protocol to identify, validate, and target functionally relevant caspases in complex tissues.

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Keywords: Immunohistochemistry Caspase-9 Retinal Tissue Confocal Microscopy Quantification Apoptosis Cell Biology Therapeutic Targets Disease Z Stack Brightness And Contrast Colocalization Vascular Marker Neuronal Areas
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