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Biochemistry

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JoVE Journal Biochemistry
Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles
 

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Article DOI: 10.3791/64360-v • 10:16 min • January 20th, 2023
January 20th, 2023 •
1, 2, 3, 3, 3, 3, 3, 2
1Central Laboratory of Women’s Hospital, Zhejiang University School of Medicine, 2School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, 3Core Facilities, Zhejiang University School of Medicine

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This protocol provides a rapid and size-specific isolation method for small extracellular vesicles by optimizing the size of the air spray nozzle, sheath fluid pressure, sample flow pressure, voltage, gain, and triggering threshold parameters.

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Keywords: Flow Cytometry Extracellular Vesicles Small Extracellular Vesicles Size-specific Sorting High-throughput Isolation Purification Flow Cytometric Sorting Parameters Forward Scatter Size Scatter Startup Procedure Laser Power Sheath Fluid Sample Flow Stream Alignment Laser Intercept Nozzle Alignment Fine Laser Alignment Rainbow Fluorescent Particles Quality Control IntelliSort
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