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Biochemistry

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JoVE Journal Biochemistry
Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers
 

Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers

Article DOI: 10.3791/64367-v • 08:11 min • August 9th, 2022
August 9th, 2022 •
*1, *1, 1
1Department of Chemistry and Henry Eyring Center for Cell & Genome Science, University of Utah
* These authors contributed equally

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résumé
Automatic Translation

The protocol presents two methods to determine the kinetics of the fluorogenic RNA aptamers Spinach2 and Broccoli. The first method describes how to measure fluorogenic aptamer kinetics in vitro with a plate reader, while the second method details the measurement of fluorogenic aptamer kinetics in cells by flow cytometry.

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Keywords: Fluorogenic RNA Aptamers In Vitro Kinetics Cellular Kinetics Real-time Studies RNA Renaturation Thermocycler Plate Reader Injector Fluorescence Measurements Binding Buffer D-FHBI
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