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Lumican Extraction from Amniotic Membrane and Determination of its Storage Temperature
 

Lumican Extraction from Amniotic Membrane and Determination of its Storage Temperature

Article DOI: 10.3791/64460-v • 04:21 min • October 14th, 2022
October 14th, 2022 •
*1, *1, 1, 1,2
1Instituto de Oftalmologia Fundacion Conde de Valenciana IAP, 2Biochemistry Department, Medicine Faculty, Universidad Nacional Autónoma de Mexico
* These authors contributed equally

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résumé
Automatic Translation

The present protocol describes the extraction of lumican from the amniotic membrane (AM) and their storing conditions as AM extract (AME) at -20 °C, 4 °C, and room temperature (RT) for 6, 12, 20, and 32 days to quantify its proteins and lumican concentration.

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Keywords: Lumican Amniotic Membrane Protein Extraction Balanced Salt Solution Dispase II De-epithelialization Liquid Nitrogen Protease Inhibitor Centrifugation Storage Temperature
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