Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Chromatin Immunoprecipitation in the Cnidarian…
JoVE Journal
Genetics
Author Produced

Ce contenu est disponible gratuitement.

JoVE Journal Genetics
Chromatin Immunoprecipitation in the Cnidarian Model System Exaiptasia diaphana
 

Chromatin Immunoprecipitation in the Cnidarian Model System Exaiptasia diaphana

Article DOI: 10.3791/64817-v • 11:48 min • March 17th, 2023
March 17th, 2023 •
*1, *2, 1, 2, 2, 1
1Red Sea Research Center (RSRC), Biological and Environmental Sciences and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST), 2KAUST Environmental Epigenetics Program (KEEP), Biological and Environmental Sciences and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST)
* These authors contributed equally

Chapitres

résumé
Automatic Translation

A chromatin immunoprecipitation (X-ChIP) assay against the histone mark H3K4me3 for the model organism Exaiptasia diaphana is presented. The specificity and effectiveness of the assay are confirmed by quantitative PCR and next-generation sequencing. This protocol enables the increased investigation of protein-DNA interactions in the sea anemone E. diaphana.

Tags

Genetics Exaiptasia Diaphana Histone Post-translational Modifications Epigenetic Modifications Chromatin Accessibility Transcriptional Machinery Environmental Stimuli ChIP-seq Protein-DNA Interactions Epigenetics Gene Regulation Applicable Protocols Symbiotic Sea Anemone Specialized ChIP Procedure Cross-linking Chromatin Extraction Immunoprecipitation Histone Mark H3K4me3 Constitutively Activated Gene Loci Quantitative PCR Next-generation Sequencing
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter