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JoVE Journal Neuroscience
In Vivo Calcium Imaging of Neuronal Ensembles in Networks of Primary Sensory Neurons in Intact Dorsal Root Ganglia
 

In Vivo Calcium Imaging of Neuronal Ensembles in Networks of Primary Sensory Neurons in Intact Dorsal Root Ganglia

Article DOI: 10.3791/64826-v • 09:07 min • February 10th, 2023
February 10th, 2023 •
1, 1, 1, 1, 1,2
1Department of Oral & Maxillofacial Surgery, School of Dentistry, University of Texas Health Science Center at San Antonio, 2Programs in Integrated Biomedical Sciences, Translational Sciences, Biomedical Engineering, Radiological Sciences, University of Texas Health Science Center at San Antonio

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This protocol describes the surgical exposure of the dorsal root ganglion (DRG) followed by GCaMP3 (genetically-encoded Ca2+ indicator; Green Fluorescent Protein-Calmodulin-M13 Protein 3) Ca2+ imaging of the neuronal ensembles using Pirt-GCaMP3 mice while applying a variety of stimuli to the ipsilateral hind paw.

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Keywords: In Vivo Calcium Imaging Neuronal Ensembles Primary Sensory Neurons Dorsal Root Ganglia Peripheral Allodynia Pain Hypersensitivity Trigeminal Ganglia Geniculate Ganglia Genetically Encoded Sensors Voltage Cell Signaling Molecules Cyclic AMP Surgical Procedure Lumbar Enlargement Transverse Process L5 DRG Isoflurane Anesthesia
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