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Isolation of Retinal Pigment Epithelial Cells from Guinea Pig Eyes
 

Isolation of Retinal Pigment Epithelial Cells from Guinea Pig Eyes

Article DOI: 10.3791/64837-v • 05:06 min • May 9th, 2023
May 9th, 2023 •
1,2,3, 4, 1
1Herbert Wertheim School Optometry and Vision Science, University of California, Berkeley, 2Department of Ophthalmology, Osaka University Graduate School of Medicine, 3Department of Ophthalmology, Tokyo Medical Center, National Hospital Organization, 4Department of Optometry and Vision Science, School of Optometry, University of Alabama at Birmingham

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résumé
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We describe a simple and efficient method for isolating cells of the retinal pigment epithelium (RPE) cells from the eyes of young pigmented guinea pigs. This procedure allows for follow-up molecular biology studies on the isolated RPE, including gene expression analyses.

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Keywords: Retinal Pigment Epithelial Cells RPE Isolation Guinea Pig Eyes Retinal Health Retinal Disease Myopia Model Biological Molecular Studies RPE Analysis Enucleation PBS Sclera Cornea Iris Ciliary Body Crystalline Lens Zonule Of Zinn Retina Choroid Sclera Tissue Storage Reagent PBS Rinse RPE Detachment Choroid Centrifugation
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