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JoVE Journal Neuroscience
Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma
 

Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma

Article DOI: 10.3791/64924-v • 10:02 min • June 2nd, 2023
June 2nd, 2023 •
*1, *1, 1, 1, 1,2, 1, 1,2, 1, 1,2
1Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, Universidad de Sevilla, 2Instituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío, CSIC, Universidad de Sevilla
* These authors contributed equally

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résumé
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The present protocol describes the purification steps and subsequent studies of four different fungal β-glucans as potential immunomodulatory molecules that enhance the anti-tumoral properties of microglia against glioblastoma cells.

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Keywords: Beta-glucan Fungal Polysaccharides Immunotherapy Glioblastoma Microglia Mushroom Extracts Pleurotus Hericium Ganoderma Soluble Mushroom Polysaccharides Insoluble Mushroom Polysaccharides Alpha-amylase Glucoamylase Alkalase Extraction Purification
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