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Isolation and Culture of Post-Natal Mouse Cerebellar Granule Neuron Progenitor Cells and Neurons
 
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Isolation and Culture of Post-Natal Mouse Cerebellar Granule Neuron Progenitor Cells and Neurons

Article DOI: 10.3791/990-v • 16:05 min • January 16th, 2009
January 16th, 2009 •
1, 2, 2,3, 2,4
1Department of Genetics and Development, Columbia University, 2Department of Pathology and Cell Biology, Columbia University, 3Department of Neuroscience, Columbia University, 4Department of Neurology, Beth Israel Deaconess Medical Center, Harvard Medical School

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Here we present a method to isolate and culture cerebellar granule neuron progenitor cells and cerebellar granule neurons from postnatal mouse.

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Isolation Culture Post-natal Mouse Cerebellar Granule Neuron Progenitor Cells Neurons Cerebellar Cortex Neuronal Properties Development Granule Neurons Purkinje Cells Inhibitory Interneurons Mammalian Brain Rodents External Granule Layer (EGL) Enrichment Purity Differentiation Proliferating Progenitor Cells Synaptogenesis Glutamate Receptor Function Interaction With Other Purified Cerebellar Cells Cell Death
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