Cynthia E. Dunbar Hematology Branch NHLBI/NIH Biography Publications Institution JoVE Articles Cynthia E. Dunbar has not added a biography. If you are Cynthia E. Dunbar and would like to personalize this page please email our Author Liaison for assistance. Publications Path to the Clinic: Assessment of IPSC-based Cell Therapies in Vivo in a Nonhuman Primate Model Cell Reports. May, 2014 | Pubmed ID: 24835994 IFN-γ Regulates Survival and Function of Tumor-induced CD11b(+) Gr-1(high) Myeloid Derived Suppressor Cells by Modulating the Anti-apoptotic Molecule Bcl2a1 European Journal of Immunology. May, 2014 | Pubmed ID: 24810636 Clonal Tracking of Rhesus Macaque Hematopoiesis Highlights a Distinct Lineage Origin for Natural Killer Cells Cell Stem Cell. Apr, 2014 | Pubmed ID: 24702997 Dynamics of HSPC Repopulation in Nonhuman Primates Revealed by a Decade-long Clonal-tracking Study Cell Stem Cell. Apr, 2014 | Pubmed ID: 24702996 Patients with Myeloid Malignancies Bearing PDGFRB Fusion Genes Achieve Durable Long-term Remissions with Imatinib Blood. Jun, 2014 | Pubmed ID: 24687085 Eltrombopag Restores Trilineage Hematopoiesis in Refractory Severe Aplastic Anemia That Can Be Sustained on Discontinuation of Drug Blood. Mar, 2014 | Pubmed ID: 24345753 No Impact of Lentiviral Transduction on Hematopoietic Stem/progenitor Cell Telomere Length or Gene Expression in the Rhesus Macaque Model Molecular Therapy : the Journal of the American Society of Gene Therapy. Jan, 2014 | Pubmed ID: 23863881 Integration-specific In Vitro Evaluation of Lentivirally Transduced Rhesus CD34(+) Cells Correlates With In Vivo Vector Copy Number Molecular Therapy. Nucleic Acids. 2013 | Pubmed ID: 24045711 Hematopoietic Stem Cell Gene Therapy:assessing the Relevance of Preclinical Models Seminars in Hematology. Apr, 2013 | Pubmed ID: 24014892 Pathophysiology and Management of Thrombocytopenia in Bone Marrow Failure: Possible Clinical Applications of TPO Receptor Agonists in Aplastic Anemia and Myelodysplastic Syndromes International Journal of Hematology. Jul, 2013 | Pubmed ID: 23690288 Defective Telomere Elongation and Hematopoiesis from Telomerase-mutant Aplastic Anemia IPSCs The Journal of Clinical Investigation. May, 2013 | Pubmed ID: 23585473 Assessing the Risks of Genotoxicity in the Therapeutic Development of Induced Pluripotent Stem Cells Molecular Therapy : the Journal of the American Society of Gene Therapy. Feb, 2013 | Pubmed ID: 23207694 Measurement of the Absolute Immature Platelet Number Reflects Marrow Production and is Not Impacted by Platelet Transfusion Transfusion. Jun, 2013 | Pubmed ID: 23043309 High Efficiency Restriction Enzyme-free Linear Amplification-mediated Polymerase Chain Reaction Approach for Tracking Lentiviral Integration Sites Does Not Abrogate Retrieval Bias Human Gene Therapy. Jan, 2013 | Pubmed ID: 22992116 An Educational Infusion: More Synergy Between Blood and the American Society of Hematology Blood. Nov, 2012 | Pubmed ID: 23197578 BCL2A1a Over-expression in Murine Hematopoietic Stem and Progenitor Cells Decreases Apoptosis and Results in Hematopoietic Transformation PloS One. 2012 | Pubmed ID: 23118966 Dynamic Clonal Analysis of Murine Hematopoietic Stem and Progenitor Cells Marked by 5 Fluorescent Proteins Using Confocal and Multiphoton Microscopy Blood. Dec, 2012 | Pubmed ID: 22995900 Thymidine Kinase Suicide Gene-mediated Ganciclovir Ablation of Autologous Gene-modified Rhesus Hematopoiesis Molecular Therapy : the Journal of the American Society of Gene Therapy. Oct, 2012 | Pubmed ID: 22910293 Eltrombopag and Improved Hematopoiesis in Refractory Aplastic Anemia The New England Journal of Medicine. Jul, 2012 | Pubmed ID: 22762314 Pharmacological Modulation of Humoral Immunity in a Nonhuman Primate Model of AAV Gene Transfer for Hemophilia B Molecular Therapy : the Journal of the American Society of Gene Therapy. Jul, 2012 | Pubmed ID: 22565846 Bone Marrow Homing and Engraftment of Human Hematopoietic Stem and Progenitor Cells is Mediated by a Polarized Membrane Domain Blood. Feb, 2012 | Pubmed ID: 22228628 Hematopoietic Stem Cell Engineering at a Crossroads Blood. Feb, 2012 | Pubmed ID: 22096239 Transient Silencing of PTEN in Human CD34(+) Cells Enhances Their Proliferative Potential and Ability to Engraft Immunodeficient Mice Experimental Hematology. Jan, 2012 | Pubmed ID: 22019626 Stem Cell Gene Therapy: the Risks of Insertional Mutagenesis and Approaches to Minimize Genotoxicity Frontiers of Medicine. Dec, 2011 | Pubmed ID: 22198747 Sox4 Cooperates with PU.1 Haploinsufficiency in Murine Myeloid Leukemia Blood. Oct, 2011 | Pubmed ID: 21878674 Insertion Sites in Engrafted Cells Cluster Within a Limited Repertoire of Genomic Areas After Gammaretroviral Vector Gene Therapy Molecular Therapy : the Journal of the American Society of Gene Therapy. Nov, 2011 | Pubmed ID: 21862999 CD9 Up-regulation on CD34+ Cells with Ingenol 3,20-dibenzoate Does Not Improve Homing in NSG Mice Blood. May, 2011 | Pubmed ID: 21617010 Patients, Hematologists, and Time Blood. Mar, 2011 | Pubmed ID: 21393501 Contributions of Gene Marking to Cell and Gene Therapies Human Gene Therapy. Jun, 2011 | Pubmed ID: 21261461 Human and Rhesus Macaque Hematopoietic Stem Cells Cannot Be Purified Based Only on SLAM Family Markers Blood. Feb, 2011 | Pubmed ID: 21163926 Ex Vivo Expansion of Retrovirally Transduced Primate CD34+ Cells Results in Overrepresentation of Clones with MDS1/EVI1 Insertion Sites in the Myeloid Lineage After Transplantation Molecular Therapy : the Journal of the American Society of Gene Therapy. Sep, 2010 | Pubmed ID: 20571542 No Evidence for Clonal Selection Due to Lentiviral Integration Sites in Human Induced Pluripotent Stem Cells Stem Cells (Dayton, Ohio). Apr, 2010 | Pubmed ID: 20166152 Gene Therapy Activates EVI1, Destabilizes Chromosomes Nature Medicine. Feb, 2010 | Pubmed ID: 20134468 Keeping Up with Blood: Introducing Our CME Program Blood. Jan, 2010 | Pubmed ID: 20110432 Repetitive Busulfan Administration After Hematopoietic Stem Cell Gene Therapy Associated with a Dominant HDAC7 Clone in a Nonhuman Primate Human Gene Therapy. Jun, 2010 | Pubmed ID: 20102258 In Vivo Selection of Hematopoietic Progenitor Cells and Temozolomide Dose Intensification in Rhesus Macaques Through Lentiviral Transduction with a Drug Resistance Gene The Journal of Clinical Investigation. Jul, 2009 | Pubmed ID: 19509470 Introducing "e-Blood" Blood. May, 2009 | Pubmed ID: 19423743 Genetically Modified CD34+ Hematopoietic Stem Cells Contribute to Turnover of Brain Perivascular Macrophages in Long-term Repopulated Primates The American Journal of Pathology. May, 2009 | Pubmed ID: 19349370 Sustained High-level Polyclonal Hematopoietic Marking and Transgene Expression 4 Years After Autologous Transplantation of Rhesus Macaques with SIV Lentiviral Vector-transduced CD34+ Cells Blood. May, 2009 | Pubmed ID: 19339698 Intercellular Transfer to Signalling Endosomes Regulates an Ex Vivo Bone Marrow Niche Nature Cell Biology. Mar, 2009 | Pubmed ID: 19198600 'Ghostbusting' at Blood Blood. Jan, 2009 | Pubmed ID: 19147793 More Frequent Blood Transfusions Blood. Jan, 2009 | Pubmed ID: 19122117 Sorting of Transgenic Secretory Proteins in Rhesus Macaque Parotid Glands After Adenovirus-mediated Gene Transfer Human Gene Therapy. Dec, 2008 | Pubmed ID: 18764738 Reduced Genotoxicity of Avian Sarcoma Leukosis Virus Vectors in Rhesus Long-term Repopulating Cells Compared to Standard Murine Retrovirus Vectors Molecular Therapy : the Journal of the American Society of Gene Therapy. Sep, 2008 | Pubmed ID: 18578011 Donor Demographic and Laboratory Predictors of Allogeneic Peripheral Blood Stem Cell Mobilization in an Ethnically Diverse Population Blood. Sep, 2008 | Pubmed ID: 18523146 HOXB4 and Retroviral Vectors: Adding Fuel to the Fire The Journal of Clinical Investigation. Apr, 2008 | Pubmed ID: 18357348 The MDS1-EVI1 Gene Complex As a Retrovirus Integration Site: Impact on Behavior of Hematopoietic Cells and Implications for Gene Therapy Molecular Therapy : the Journal of the American Society of Gene Therapy. Mar, 2008 | Pubmed ID: 18227842 The Yin and Yang of Stem Cell Gene Therapy: Insights into Hematopoiesis, Leukemogenesis, and Gene Therapy Safety Hematology / the Education Program of the American Society of Hematology. American Society of Hematology. Education Program. 2007 | Pubmed ID: 18024665 Relapse Following Discontinuation of Imatinib Mesylate Therapy for FIP1L1/PDGFRA-positive Chronic Eosinophilic Leukemia: Implications for Optimal Dosing Blood. Nov, 2007 | Pubmed ID: 17709602 Transduction of Rhesus Macaque Hematopoietic Stem and Progenitor Cells with Avian Sarcoma and Leukosis Virus Vectors Human Gene Therapy. Aug, 2007 | Pubmed ID: 17655493 No Evidence of Clonal Dominance in Primates Up to 4 Years Following Transplantation of Multidrug Resistance 1 Retrovirally Transduced Long-term Repopulating Cells Stem Cells (Dayton, Ohio). Oct, 2007 | Pubmed ID: 17615269 Antibody-mediated Cell Labeling of Peripheral T Cells with Micron-sized Iron Oxide Particles (MPIOs) Allows Single Cell Detection by MRI Contrast Media & Molecular Imaging. May-Jun, 2007 | Pubmed ID: 17541955 Hematopoietic Stem-cell Behavior in Nonhuman Primates Blood. Sep, 2007 | Pubmed ID: 17526860 Keratinocyte Growth Factor Augments Immune Reconstitution After Autologous Hematopoietic Progenitor Cell Transplantation in Rhesus Macaques Blood. Jul, 2007 | Pubmed ID: 17374737 Cytokine-independent Growth and Clonal Expansion of a Primary Human CD8+ T-cell Clone Following Retroviral Transduction with the IL-15 Gene Blood. Jun, 2007 | Pubmed ID: 17353346 Adeno-associated Virus Serotype 2-mediated Gene Transfer to the Parotid Glands of Nonhuman Primates Human Gene Therapy. Feb, 2007 | Pubmed ID: 17328682 Correction of the Disease Phenotype in Canine Leukocyte Adhesion Deficiency Using Ex Vivo Hematopoietic Stem Cell Gene Therapy Blood. Nov, 2006 | Pubmed ID: 16868255 Genotoxicity of Retroviral Integration in Hematopoietic Cells Molecular Therapy : the Journal of the American Society of Gene Therapy. Jun, 2006 | Pubmed ID: 16624621 In Vitro Culture During Retroviral Transduction Improves Thymic Repopulation and Output After Total Body Irradiation and Autologous Peripheral Blood Progenitor Cell Transplantation in Rhesus Macaques Stem Cells (Dayton, Ohio). Jun, 2006 | Pubmed ID: 16497945 Combination Therapy with RFVIIa and Platelets for Hemorrhage in Patients with Severe Thrombocytopenia and Alloimmunization American Journal of Hematology. Mar, 2006 | Pubmed ID: 16493611 Anti-interleukin-2 Receptor Antibody (daclizumab) Treatment of Corticosteroid-refractory Autoimmune Thrombocytopenic Purpura Haematologica. Feb, 2006 | Pubmed ID: 16461323 AMD3100 Mobilizes Hematopoietic Stem Cells with Long-term Repopulating Capacity in Nonhuman Primates Blood. May, 2006 | Pubmed ID: 16439684 Mobilization As a Preparative Regimen for Hematopoietic Stem Cell Transplantation Blood. May, 2006 | Pubmed ID: 16439683 Acute Myeloid Leukemia is Associated with Retroviral Gene Transfer to Hematopoietic Progenitor Cells in a Rhesus Macaque Blood. May, 2006 | Pubmed ID: 16439674 Large Animal Models for Stem and Progenitor Cell Analysis Current Protocols in Immunology / Edited by John E. Coligan ... [et Al.]. Nov, 2005 | Pubmed ID: 18432946 Outcomes and Risks of Granulocyte Colony-stimulating Factor in Patients with Coronary Artery Disease Journal of the American College of Cardiology. Nov, 2005 | Pubmed ID: 16256862 Hematopoietic Stem Cell Gene Therapy: Dead or Alive? Trends in Biotechnology. Dec, 2005 | Pubmed ID: 16216357 A New Direction for Gene Therapy: Intrathymic T Cell-specific Lentiviral Gene Transfer The Journal of Clinical Investigation. Aug, 2005 | Pubmed ID: 16075048 Large Granular Lymphocytic Proliferation-associated Cyclic Thrombocytopenia American Journal of Hematology. Aug, 2005 | Pubmed ID: 16044437 Stem Cell Gene Transfer: Insights into Integration and Hematopoiesis from Primate Genetic Marking Studies Annals of the New York Academy of Sciences. Jun, 2005 | Pubmed ID: 15958711 Recurrent Retroviral Vector Integration at the Mds1/Evi1 Locus in Nonhuman Primate Hematopoietic Cells Blood. Oct, 2005 | Pubmed ID: 15933056 Imatinib Inhibits T-cell Receptor-mediated T-cell Proliferation and Activation in a Dose-dependent Manner Blood. Mar, 2005 | Pubmed ID: 15572591 Low-dose Total Body Irradiation Causes Clonal Fluctuation of Primate Hematopoietic Stem and Progenitor Cells Blood. Feb, 2005 | Pubmed ID: 15383461 Distinct Genomic Integration of MLV and SIV Vectors in Primate Hematopoietic Stem and Progenitor Cells PLoS Biology. Dec, 2004 | Pubmed ID: 15550989 Genetic Manipulation of Hematopoietic Stem Cells Seminars in Hematology. Oct, 2004 | Pubmed ID: 15508111 Gene Therapy for Hematologic Disease: Don't Throw the Baby out with the Bathwater! Seminars in Hematology. Oct, 2004 | Pubmed ID: 15508110 MRI Detection of Single Particles for Cellular Imaging Proceedings of the National Academy of Sciences of the United States of America. Jul, 2004 | Pubmed ID: 15256592 The Impact of Low-dose Busulfan on Clonal Dynamics in Nonhuman Primates Blood. Sep, 2004 | Pubmed ID: 15126320 The Presence of the Carboxy-terminal Fragment of Fibronectin Allows Maintenance of Non-human Primate Long-term Hematopoietic Repopulating Cells During Extended Ex Vivo Culture and Transduction Experimental Hematology. Feb, 2004 | Pubmed ID: 15102477 In Vivo Gene Marking of Rhesus Macaque Long-term Repopulating Hematopoietic Cells Using a VSV-G Pseudotyped Versus Amphotropic Oncoretroviral Vector The Journal of Gene Medicine. Apr, 2004 | Pubmed ID: 15079811 Long-term Clinical and Molecular Follow-up of Large Animals Receiving Retrovirally Transduced Stem and Progenitor Cells: No Progression to Clonal Hematopoiesis or Leukemia Molecular Therapy : the Journal of the American Society of Gene Therapy. Mar, 2004 | Pubmed ID: 15006605 Efficient Gene Transfer into Rhesus Repopulating Hematopoietic Stem Cells Using a Simian Immunodeficiency Virus-based Lentiviral Vector System Blood. Jun, 2004 | Pubmed ID: 14976042 Effect of Chronic Cytokine Therapy on Clonal Dynamics in Nonhuman Primates Blood. Jun, 2004 | Pubmed ID: 14962906 Transient in Vivo Selection of Transduced Peripheral Blood Cells Using Antifolate Drug Selection in Rhesus Macaques That Received Transplants with Hematopoietic Stem Cells Expressing Dihydrofolate Reductase Vectors Blood. Feb, 2004 | Pubmed ID: 12920024 Resetting the Immune System in Immune Thrombocytopenic Purpura: Immunoablative Strategies Clinical Advances in Hematology & Oncology : H&O. Jun, 2003 | Pubmed ID: 16235418 Direct Comparison of RD114-pseudotyped Versus Amphotropic-pseudotyped Retroviral Vectors for Transduction of Rhesus Macaque Long-term Repopulating Cells Molecular Therapy : the Journal of the American Society of Gene Therapy. Oct, 2003 | Pubmed ID: 14529834 Serial Cardiac Magnetic Resonance Imaging of Injected Mesenchymal Stem Cells Circulation. Aug, 2003 | Pubmed ID: 12912822 Efficient Characterization of Retro-, Lenti-, and Foamyvector-transduced Cell Populations by High-accuracy Insertion Site Sequencing Annals of the New York Academy of Sciences. May, 2003 | Pubmed ID: 12799289 Highly Efficient Endosomal Labeling of Progenitor and Stem Cells with Large Magnetic Particles Allows Magnetic Resonance Imaging of Single Cells Blood. Aug, 2003 | Pubmed ID: 12676779 Prolonged Multilineage Clonal Hematopoiesis in a Rhesus Recipient of CD34 Positive Cells Marked with a RD114 Pseudotyped Oncoretroviral Vector Blood Cells, Molecules & Diseases. Jan-Feb, 2003 | Pubmed ID: 12667996 Persistence and Expression of the Adenosine Deaminase Gene for 12 Years and Immune Reaction to Gene Transfer Components: Long-term Results of the First Clinical Gene Therapy Trial Blood. Apr, 2003 | Pubmed ID: 12456496 Retroviral Transduction Efficiency of G-CSF+SCF-mobilized Peripheral Blood CD34+ Cells is Superior to G-CSF or G-CSF+Flt3-L-mobilized Cells in Nonhuman Primates Blood. Mar, 2003 | Pubmed ID: 12424191 High-dose Cyclophosphamide with Autologous Lymphocyte-depleted Peripheral Blood Stem Cell (PBSC) Support for Treatment of Refractory Chronic Autoimmune Thrombocytopenia Blood. Jan, 2003 | Pubmed ID: 12393623 Update on Hematopoietic Stem Cell Gene Transfer Using Non-human Primate Models Current Opinion in Molecular Therapeutics. Oct, 2002 | Pubmed ID: 12435050 Polyclonal Long-term Repopulating Stem Cell Clones in a Primate Model Blood. Oct, 2002 | Pubmed ID: 12351380 Absence of Donor-derived Keratinocyte Stem Cells in Skin Tissues Cultured from Patients After Mobilized Peripheral Blood Hematopoietic Stem Cell Transplantation Experimental Hematology. Aug, 2002 | Pubmed ID: 12160846 Activity of STI571 in Chronic Myelomonocytic Leukemia with a Platelet-derived Growth Factor Beta Receptor Fusion Oncogene Blood. Aug, 2002 | Pubmed ID: 12130532 Plasticity and Hematopoiesis: Circe's Transforming Potion? Current Opinion in Hematology. Jul, 2002 | Pubmed ID: 12042699 Genetic Marking As an Approach to Studying in Vivo Hematopoiesis: Progress in the Non-human Primate Model Oncogene. May, 2002 | Pubmed ID: 12032769 Retroviral Transduction and Engraftment Ability of Primate Hematopoietic Progenitor and Stem Cells Transduced Under Serum-free Versus Serum-containing Conditions Molecular Therapy : the Journal of the American Society of Gene Therapy. Mar, 2002 | Pubmed ID: 11863422 In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy Daniela Malide1, Jean-Yves Métais2, Cynthia E. Dunbar2 1Light Microscopy Core Facility, NHLBI/NIH, 2Hematology Branch, NHLBI/NIH JoVE 51669 Biologia
In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy Daniela Malide1, Jean-Yves Métais2, Cynthia E. Dunbar2 1Light Microscopy Core Facility, NHLBI/NIH, 2Hematology Branch, NHLBI/NIH JoVE 51669 Biologia