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In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy
 

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

Article DOI: 10.3791/51669-v • 17:08 min • August 6th, 2014
August 6th, 2014 •
1, 2, 2
1Light Microscopy Core Facility, NHLBI/NIH, 2Hematology Branch, NHLBI/NIH

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Combinatorial 5 fluorescent proteins marking of hematopoietic stem and progenitor cells allows in vivo clonal tracking via confocal and two-photon microscopy, providing insights into bone marrow hematopoietic architecture during regeneration. This method allows non-invasive fate mapping of spectrally-coded HSPCs-derived cells in intact tissues for extensive periods of time following transplantation.

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In Vivo Clonal Tracking Hematopoietic Stem Cells Progenitor Cells Fluorescent Proteins Confocal Microscopy Multiphoton Microscopy Hematopoiesis Bone Marrow Transplant Lentiviral Vectors Cell Fate Mapping Advanced Microscopy Imaging Cerulean EGFP Venus TdTomato MCherry Color Marked Cells High Resolution Assessment Tissue Structure Volumetric Analyses Non-invasive Detection Live Studies Video-rate Multiphoton Imaging
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