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Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer
 

Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer

Article DOI: 10.3791/63045-v • 10:17 min • September 24th, 2021
September 24th, 2021 •
1, 2, 2,3,4, 1,4,5
1Department of Pathology, University of Michigan, Ann Arbor, 2Department of Internal Medicine, University of Michigan, Ann Arbor, 3Department of Cellular and Developmental Biology, University of Michigan, Ann Arbor, 4Rogel Cancer Center, University of Michigan, Ann Arbor, 5Institute of Gerontology, University of Michigan, Ann Arbor

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The method presented here summarizes optimized protocols for assessing cellular bioenergetics in non-adherent mouse hematopoietic stem and primitive progenitor cells (HSPCs) using the extracellular flux analyzer to measure the extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) of HSPCs in real time.

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Keywords: Hematopoietic Stem Cells Hematopoiesis Glycolysis Oxidative Phosphorylation Cellular Bioenergetics Extracellular Flux Analyzer Metabolic Switch Mouse Hematopoietic Stem And Progenitor Cells High Throughput Quantification Metabolic Pathways Pluripotency Suspension Cells Cell Adhesive Solution 96-well Microplate
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