Tracing <em>de novo</em> Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS

Katherine D. Castro; Lilian Valadares Tose; Matthew Willetts; Melvin A. Park; Marcela Nouzova; Fernando G. Noriega; Francisco Fernandez-Lima

doi:10.3791/65590

Screening Lipid Structures Using Stable Isotope Labeling

August 23, 2024

De novo lipiden traceren met behulp van stabiele isotopenetikettering LC-TIMS-TOF MS/MS

00:47 min

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Calibration of the Mass Spectrometer for Screening Lipids with Stable Isotope Labeling by LC-TIMS-TOF MS/MS

02:50 min

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LC-TIMS-TOF MS/MS Data Analysis for Screening Lipids with Stable Isotope Labeling

03:39 min

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Katherine D. Castro, Lilian Valadares Tose, Matthew Willetts, Melvin A. Park, Marcela Nouzova, Fernando G. Noriega⁵, Francisco Fernandez-Lima⁶

¹Deparment of Chemistry and Biochemistry,Florida International University, ²Bruker Daltonics Inc., ³Institute of Parasitology,Biology Centre CAS, ⁴Department of Biological Sciences,Florida International University, ⁵Department of Parasitology,University of South Bohemia, ⁶Biomolecular Sciences Institute,Florida International University

Summary

Hier wordt een methode gepresenteerd voor het screenen van lipidestructuren door middel van stabiele isotopenlabeling door gebruik te maken van hun retentietijd, mobiliteit en fragmentatie.

Screening Lipid Structures Using Stable Isotope Labeling

De novo lipiden traceren met behulp van stabiele isotopenetikettering LC-TIMS-TOF MS/MS

Calibration of the Mass Spectrometer for Screening Lipids with Stable Isotope Labeling by LC-TIMS-TOF MS/MS

LC-TIMS-TOF MS/MS Data Analysis for Screening Lipids with Stable Isotope Labeling

Summary

Article