Materials
Name | Company | Catalog Number | Comments |
Hepes | Sigma-Aldrich | H3375 | |
NaOH | Fischer-Scientific | S320-1 | |
Sorbitol | Sigma-Aldrich | S1876 | |
EDTA | Fischer-Biotech | BP121 | |
MnCl2 | Sigma-Aldrich | 221279 | |
MgCl2 | Sigma-Aldrich | M0250 | |
KCl | Sigma-Aldrich | P3911 | |
NaCl | Sigma-Aldrich | S9625 | |
Laser Scanning Confocal Microscope | Carl Zeiss Inc | Model: LSM710 | |
Carboxyfluorescein diacetate (CFDA) | Sigma-Aldrich | 21879 | |
Dimethyl sulfoxide (DMSO) | EMD | MX1458-6 | |
Waring blender | Waring | Model: 31BL92 | |
Fiji | fiji.sc | Open-source software for analyzing biological images |
References
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