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Antibody Profiling by Luciferase Immunoprecipitation Systems (LIPS)
 
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Antibody Profiling by Luciferase Immunoprecipitation Systems (LIPS)

Article DOI: 10.3791/1549-v • 12:20 min • October 7th, 2009
October 7th, 2009 •
1, 1, 1, 1
1Neurobiology and Pain Therapeutics Section, National Institute of Dental and Craniofacial Research, National Institutes of Health

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The technical aspects of performing LIPS (Luciferase Immunoprecipitation Systems) are described. The overall approach involves expressing chimeric genes encoding antigens fused to Renilla luciferase (Ruc) in mammalian cells. Crude Ruc-antigen extracts are then prepared and, without purification, employed in immunoprecipitation assays to quantify antibodies.

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Antibody Profiling Luciferase Immunoprecipitation Systems (LIPS) Comprehensively Understanding Quantifying Antibody Profiles Autoantigens Infectious Agents Disease Mechanisms Markers Substratify Disease Clinical Features Pathogenesis Highly Quantitative Method Patient Sera Antibody Responses Pathogen Antigens Infection ELISAs Humoral Serological Response Profiles Universal Format Dynamic Antibody Titer Ranges Proteome HIV Informative Single Antigen ELISA Purified Antigens Assay Optimization Steps
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