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JoVE Journal Neuroscience
Mechanical Manipulation of Neurons to Control Axonal Development
 

Mechanical Manipulation of Neurons to Control Axonal Development

Article DOI: 10.3791/2509-v • 10:03 min • April 10th, 2011
April 10th, 2011 •
1, 1, 1
1Department of Zoology, Michigan State University, East Lansing

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Application and direct measurements of forces on neurons in the 2-1000 microdyne range are achieved with high precision using calibrated glass needles. This methodology can be used to control and measure several aspects of axonal development, including axonal initiation, axonal tension, velocity of axonal elongation, and force vectors.

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Mechanical Manipulation Neurons Control Axonal Development Glass Micro-fibers Forces Hookean Bending Calibration Equipment Requirements Procedures Fabricating Treating Cells Force Regimes Cell Types Flexibility Methodology Investigation Visualization Direct Intervention Cellular Events Stimulation Regulation Axonal Growth Retraction Detachment
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