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Capitoli
- 00:05Titolo
- 01:19UV Cross-linking of Tissue Culture Cells
- 02:10Bead Preparation
- 02:53Cell Lysis and Partial RNA Digestion
- 03:47Immunoprecipitation
- 04:26RNA 5′ End Labelling
- 05:08SDS-PAGE and Membrane Transfer
- 06:27Gel Purification of cDNA
- 08:44Representative Results
- 10:00Conclusion
La disposición espacial de ARN-proteínas de unión en una transcripción es un determinante clave de la regulación post-transcripcional. Por lo tanto, hemos desarrollado cada nucleótido reticulación UV resolución y inmunoprecipitación (iClip) que permite que precisa todo el genoma de mapeo de los sitios de unión de una proteína de unión al ARN.
Tags
ICLIPTranscriptome-wide MappingProtein-RNA InteractionsIndividual Nucleotide ResolutionRNA-binding ProteinsPost-transcriptional RegulationPositional InformationBinding SitesBiochemical MethodsNative Cellular ContextAffinity PurificationImmunoprecipitationDifferential DisplayMicroarray AnalysisRIP-CHIPIndirect InteractionsNon-physiological InteractionsCLIP (UV Cross-linking And Immunoprecipitation)UV Cross-linkingRigorous Purification SchemesDenaturing Polyacrylamide Gel ElectrophoresisHigh-throughput Sequencing TechnologiesHITS-CLIPCLIP-seqPAR-CLIPPhotoreactive Ribonucleoside Analogs
