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Immunology and Infection

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JoVE Journal Immunology and Infection
Immuno Assay-fluorescentie van Leptospiral Surface-blootgesteld Eiwitten
 
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Immuno Assay-fluorescentie van Leptospiral Surface-blootgesteld Eiwitten

Article DOI: 10.3791/2805-v • 10:47 min • July 1st, 2011
July 1st, 2011 •
1,2, 3,4
1Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, 2Research service, 151, Veterans Affairs Greater Los Angeles Healthcare System, 3Departments of Medicine, Urology at David Geffen School of Medicine and Department of Microbiology, Immunology and Molecular Gentics, University of California Los Angeles (UCLA), 4Division of Infectious Diseases, 111F, Veterans Affairs Greater Los Angeles Health Care System

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Een efficiënte methode om het oppervlak-blootstelling van leptospiral eiwitten te bepalen is beschreven. De methode is speciaal ontworpen om verstoring van de kwetsbare buitenste membraan van leptospiral cellen te voorkomen. Deze techniek vergt inzet van een aantal negatieve controles te beoordelen van de integriteit van de buitenste membraan en de specificiteit van de antistof reactie.

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Immunologie Moleculaire Biologie Leptospira intacte cellen buitenste membraan oppervlakte-eiwitten worden blootgesteld oppervlakte immuno-fluorescentie
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