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Utføre Custom mikroRNA Microarray Experiments
 
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Utføre Custom mikroRNA Microarray Experiments

Article DOI: 10.3791/3250-v • 07:05 min • October 28th, 2011
October 28th, 2011 •
1, 1,2
1Department of Pharmacology, University of Minnesota, 2Masonic Cancer Center, University of Minnesota

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En enkel prosedyre å utføre tilpassede mikroRNA microarray eksperimenter er beskrevet. Trinnene omfatter isolere RNA, merking RNA og referanse DNA, hybridizing prøvene til microarrays, skanning microarrays, kvantifisere og analysere hybridisering signaler.

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Molecular Biology genetikk mikroRNA tilpasset microarray oligonukleotid sonder RNA merking
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