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Immunology and Infection
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JoVE Journal Immunology and Infection
Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
 

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Article DOI: 10.3791/3344-v • 11:09 min • October 23rd, 2011
October 23rd, 2011 •
1, 1, 2, 2, 3
1Saskatoon Research Centre, Agriculture and Agri-Food Canada, 2Department of Veterinary Microbiology, University of Saskatchewan, 3Plant Biotechnology Institute, National Research Council of Canada

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We describe a multiplex method for the detection of microorganisms within a sample using oligonucleotide-coupled fluorescent beads. Amplicon from all organisms within a sample is hybridized to a panel of probe-coupled beads. A Luminex or Bio-Plex instrument is used to query each bead for bead type and hybridization signal.

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Multiplex Detection Bacteria Clinical Samples Environmental Samples Oligonucleotide-coupled Fluorescent Microspheres Bacterial Vaginosis Polymicrobial Syndrome Lactobacillus Gardnerella Vaginalis Atopobium Vaginae Negative Health Outcomes HIV Acquisition Clinical Management Diagnosis Gram Stains Vaginal Swab Smears Subjective Interpretations Vaginal Microbiota Composition Deep Sequencing Molecular Diagnosis
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