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Immunology and Infection

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JoVE Journal Immunology and Infection
Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
 

Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma

Article DOI: 10.3791/58711-v • 07:52 min • January 9th, 2019
January 9th, 2019 •
*1,2, *3, 3, 2, 2, 2, 4, 4, 2,3, 2,4
1Department of Laboratory Medicine, AnSteel Group General Hospital, 2Cancer Science Institute of Singapore, National University of Singapore, 3Department of Pathology, Yong Loo Lin School of Medicine, National University of Singapore, 4Department of Haematology-Oncology, National University Health System
* These authors contributed equally

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Here we describe a protocol for multiplex fluorescent immunohistochemical staining and imaging for the simultaneous localization of multiple cancer-associated antigens in lymphoma. This protocol can be extended to the colocalization analysis of biomarkers within all tissue sections.

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Multiplexed Fluorescent Immunohistochemical Staining Imaging Analysis Histological Samples Lymphoma Chromogenic Immunohistochemistry Tumor Cells Microenvironment Standardizing Scoring Antibody-based Staining Spectral Imaging Antigen Co-expression Spatial Relationship Clinical Material De-waxed Slides Rehydrated Slides Antigen Retrieval Buffer Microwave-induced Epitope Retrieval Microwave Stripping Staining Process Forceps Heat-proof Gloves
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