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Bioengineering

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JoVE Journal Bioengineering
Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers
 
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Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers

Article DOI: 10.3791/3405-v • 08:48 min • October 13th, 2011
October 13th, 2011 •
1, 2, 2
1LSA Biophysics, University of Michigan, 2LSA Biophysics, Department of Physics, University of Michigan

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We illustrate the use of a constant force axial optical tweezers to explore the mechanical properties of short DNA molecules. By stretching DNA axially, we minimize steric hindrances and artifacts arising in conventional lateral manipulation, allowing us to study DNA molecules as short as ~100 nm.

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Stretching Short Sequences DNA Constant Force Axial Optical Tweezers Single-molecule Techniques Experimental Difficulties Biological Events Histone Binding Protein-mediated Looping Mechanical Properties Packaging Of DNA Compact Nucleosomes Chromatin Fibers Practical Guide Optical Tweezing Technique Contour Lengths Submicron Length DNA Bead Coverslip Axial Position Steric Effects Microspheres
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