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Journal / Bioengineering / Tracking Hypoxic Signaling within Encapsulated…
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Bioengineering

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JoVE Journal Bioengineering
Tracking Hypoxic Signaling within Encapsulated Cell Aggregates
 

Tracking Hypoxic Signaling within Encapsulated Cell Aggregates

Article DOI: 10.3791/3521-v • 09:14 min • December 16th, 2011
December 16th, 2011 •
1, 1, 2
1Biomedical Engineering Program, University of South Carolina, 2Chemical Engineering Department, University of South Carolina

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A method for photo-encapsulation of cells in a crosslinked PEG hydrogel is described. Hypoxic signaling within encapsulated murine insulinoma (MIN6) aggregates is tracked using a fluorescent marker system. This system allows serial examination of cells within a hydrogel scaffold and correlation of hypoxic signaling with changes in cell phenotype.

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Tracking Hypoxic Signaling Encapsulated Cell Aggregates Diabetes Mellitus Type 1 Pancreatic Beta-cells Insulin Production Hyperglycemia Exogenous Insulin Injection Transplantation Functional Pancreatic Tissue Normoglycemia Immune System Encapsulation Biologically-derived Materials Alginate Agarose Inflammation Fibrotic Overgrowth Nutrient Transport Oxygen Transport Synthetic Poly(ethylene Glycol) PEG-based Hydrogels Photo-crosslinking Reaction UV Degradation Photoinitiator Irgacure 2959 Free Radicals
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