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Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
 

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids

Article DOI: 10.3791/3583-v • 07:27 min • January 26th, 2012
January 26th, 2012 •
1, 2, 1, 1, 3, 1
1Department of Energy, Environmental and Chemical Engineering, Washington University, 2Department of Biology, Washington University, 3Department of Energy, Environmental and Chemical Engineering and Department of Biology, Washington University

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13C-isotope labeling is a useful technique for determining the cell central metabolism for various types of microorganisms. After cells have been cultured with a specific labeled substrate, GC-MS measurement can reveal functional metabolic pathways based on unique labeling patterns in proteinogenic amino acids.

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Metabolic Pathway Confirmation Metabolic Pathway Discovery 13C Labeling Proteinogenic Amino Acids Biochemistry Functional Genomics 13C-assisted Metabolism Analysis Isotopic Labeling Atom Transition Paths Biochemical Network Transcriptomics Proteomics Isotopomer-assisted Analysis Of Metabolic Pathways Labeled Substrates Minimal Medium Carbon Source Measurement Noises Non-labeled Carbon Nutrient Supplements Labeled Substrate Choice Reaction Stereochemistry Intermediate Products Singly Labeled Carbon Substrates Uniformly Labeled Substrates Amino Acid Labeling Patterns GC-MS
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