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检测使用荧光脱氧核酶的细菌
JoVE Journal
Immunologia e infezioni
This content is Free Access.
JoVE Journal Immunologia e infezioni
Detection of Bacteria Using Fluorogenic DNAzymes
DOI:

13:20 min

May 28, 2012

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Capitoli

  • 00:05Titolo
  • 02:02Construction of RFD-EC1 and RFSS1 by Template Mediated Enzymatic Ligation
  • 04:38Detection using Fluorescence Spectrophotometer
  • 06:28Detection by Gel Electrophoresis
  • 08:04Single Cell Detection
  • 09:55Experimental Validation using E. coli Crude Extracellular Mixtures
  • 12:42Conclusion

Summary

Traduzione automatica

我们最近报道一种新的方法,产生荧光脱氧核酶探针,可用于设立一个简单的“混合和阅读的”细菌检测荧光法。这些特殊的DNA探针,催化裂解1生色修饰DNA-RNA的存在原油外的一个特定的细菌,从而转化为细菌检测荧光信号的产生,产生的混合物(CEM)的嵌合基板。在这份报告中,我们将描述模型的细菌检测采用特定的核酶探针记“RFD的EC1的”为重点的实验程序,<em>大肠埃希氏菌(大肠杆菌)</em>。

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