Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / In Vitro Analysis of PDZ-dependent CFTR…
JoVE Journal
Biology

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Biology
In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes
 

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes

Article DOI: 10.3791/4091-v • 10:05 min • August 13th, 2012
August 13th, 2012 •
1, 1, 1,2,3
1Department of Biochemistry & Molecular Biology, Wayne State University School of Medicine, 2Cardiovascular Research Institute, Wayne State University School of Medicine, 3Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine

Capitoli

Riepilogo
Automatic Translation

Cystic fibrosis transmembrane conductance regulator (CFTR), an epithelial chloride channel, has been reported to interact with various proteins and regulate important cellular processes; among them the CFTR PDZ motif-mediated interactions have been well documented. This protocol describes methods we developed to assemble a PDZ-dependent CFTR macromolecular signaling complex in vitro.

Tags

In Vitro Analysis PDZ-dependent CFTR Macromolecular Signaling Complexes CFTR Chloride Channel Transepithelial Fluid Homeostasis Cystic Fibrosis Secretory Diarrhea CFTR Cl- Channel Physical Interactions Functional Interactions Transporters Ion Channels Receptors Kinases Phosphatases Signaling Molecules Cytoskeletal Elements CFTR Carboxyl Terminal Tail PSD95/Dlg1/ZO-1 (PDZ) Motif Scaffold Proteins
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter