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Overvåking Cell-autonome biologiske klokke Rhythms of Gene Expression Bruke luciferase Bioluminescens Reporters
 
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Overvåking Cell-autonome biologiske klokke Rhythms of Gene Expression Bruke luciferase Bioluminescens Reporters

Article DOI: 10.3791/4234-v • 10:38 min • September 27th, 2012
September 27th, 2012 •
1, 1, 1, 1, 1
1Department of Biological Sciences, The University of Memphis

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Fungere biologiske klokka innenfor enkelte cellene, dvs. de er celle-autonomt. Her beskriver vi metoder for generering celle-autonome klokke modeller med ikke-invasiv, luciferase-baserte sanntids bioluminescens teknologi. Reporter celler gir medgjørlig, funksjonelle modellsystem for å studere circadian biologi.

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Genetikk Molecular Biology cellebiologi kjemisk biologi biologiske klokke firefly luciferase real-time Bioluminescens teknologi celle-autonome modell lentiviral vektor RNA-interferens (RNAi) high-throughput screening (HTS)
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