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Journal / Immunology and Infection / Artificial Antigen Presenting Cell (aAPC)…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Artificial Antigen Presenting Cell (aAPC) Mediated Activation and Expansion of Natural Killer T Cells
 

Artificial Antigen Presenting Cell (aAPC) Mediated Activation and Expansion of Natural Killer T Cells

Article DOI: 10.3791/4333-v • 13:18 min • December 29th, 2012
December 29th, 2012 •
*1, *1, 1
1Department of Microbiology and Immunology, University of Maryland
* These authors contributed equally

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Here we describe a method for activating and expanding human NKT cells from bulk T cell populations using artificial antigen presenting cells (aAPC). The use of CD1d-based aAPC provides a standardized method for generating high numbers of functional NKT cells.

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Artificial Antigen Presenting Cell (aAPC) Natural Killer T (NKT) Cells Lipid Antigen CD1 Molecules Invariant TCRα Chain Canonical NKT (iNKT) Cells CD4-CD8- Thymic Precursor T Cells α-Galactosylceramide (α-GalCer) Cytokines Adoptive Immunotherapy Autologous Antigen Presenting Cells Monocyte-derived DC Costimulatory Molecules
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