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Journal / Immunology and Infection / Analysis of Human Natural Killer Cell Metabolism
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Immunology and Infection

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JoVE Journal Immunology and Infection
Analysis of Human Natural Killer Cell Metabolism
 

Analysis of Human Natural Killer Cell Metabolism

Article DOI: 10.3791/61466-v • 09:03 min • June 22nd, 2020
June 22nd, 2020 •
1,2, 3, 3
1Cardiovascular Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 2Departamento de Biología Molecular, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid (CSIC-UAM), 3Lymphoid Malignancies Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health

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In this paper, we describe a method to measure glycolysis and mitochondrial respiration in primary human Natural Killer (NK) cells isolated from peripheral blood, at rest or following IL15-induced activation. The protocol described could be easily extended to primary human NK cells activated by other cytokines or soluble stimuli.

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Human Natural Killer Cell Metabolism Activation Oxygen Consumption PH Changes Extracellular Flux Analyzer High Throughput Screens Glycolysis Respiration Lymphocyte Separation Medium Blood Centrifuge Mononuclear Cells PBS NK Separation Buffer
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