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Application of an In vitro DNA Protection Assay to Visualize Stress Mediation Properties of the Dps Protein
 

Application of an In vitro DNA Protection Assay to Visualize Stress Mediation Properties of the Dps Protein

Article DOI: 10.3791/50390-v • 12:41 min • May 31st, 2013
May 31st, 2013 •
1, 1, 1
1Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology

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The DNA-binding protein from starved cells (Dps) plays a crucial role in combating bacterial stress. This article discusses the purification of E. coli Dps and the protocol for an in vitro assay demonstrating Dps-mediated protection of DNA from degradation by reactive oxygen species.

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In Vitro DNA Protection Assay Stress Mediation Properties Dps Protein Oxidative Stress Aerobic Life Fenton Chemistry DNA Degradation Intracellular Radicals DNA Repair Quantitative Technique Radical-mediated DNA Damage Protective Properties Of Proteins Or Chemicals Gel Electrophoresis DNA-binding Protein From Starved Cells (Dps) Mini-ferritin Environmental Stressors
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