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JoVE Journal
Biology

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The Assembly and Application of 'Shear Rings'
 

The Assembly and Application of 'Shear Rings': A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress

Article DOI: 10.3791/54632-v 09:20 min October 31st, 2016
October 31st, 2016

Capitoli

Riepilogo

Different levels and patterns of fluid shear are known to modulate endothelial gene expression, phenotype and susceptibility to disease. We discuss the assembly and use of 'shear rings': a model that produces unidirectional, periodic shear stress patterns. Shear rings are simple to assemble, economical and can produce high cell yields.

Tags

Keywords: Shear Rings Endothelial Model Fluid Flow Shear Stress Tissue Culture Device Methylene Chloride Petri Dish Adhesion Periodic Flow
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