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Immunology and Infection

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JoVE Journal Immunology and Infection
A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells
 

A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells

Article DOI: 10.3791/50606-v • 16:04 min • June 19th, 2013
June 19th, 2013 •
1, 1, 1, 1
1Mork Family Department of Chemical Engineering and Materials Science, Viterbi School of Engineering, University of Southern California, Los Angeles

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Automatic Translation

Here, we use retroviral transduction and concatemeric transfection to create a cell line that can express the components of a lentiviral vector (LV) in the absence of tetracycline. This LV encodes GFP and is pseudotyped with a glycoprotein, SVGmu, which is specific for a receptor on dendritic cells.

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Tetracycline-regulated Cell Line High-titer Lentiviral Vectors Dendritic Cells LV Production Self-inactivating LVs GPR Cell Line GPRS Cell Line Viral Components Dendritic Cell-specific Glycoprotein SVGmu Concatemeric DNA Transfection LV Transfer Plasmid Reporter Gene GFP Selectable Marker Transient Transfection Transduction Efficiency T Cell Immune Response LV-based Vaccines Clinical Studies Cancer Infectious Diseases
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