Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / Improved Method for the Preparation of a Human…
JoVE Journal
Bioengineering

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Bioengineering
Improved Method for the Preparation of a Human Cell-based, Contact Model of the Blood-Brain Barrier
 

Improved Method for the Preparation of a Human Cell-based, Contact Model of the Blood-Brain Barrier

Article DOI: 10.3791/50934-v • 08:40 min • November 12th, 2013
November 12th, 2013 •
1, 1
1Australian Centre for Blood Diseases, Monash University

Capitoli

Riepilogo
Automatic Translation

Establishment of human models of the blood-brain barrier (BBB) can benefit research into brain conditions associated with BBB failure. We describe here an improved technique for preparation of a contact BBB model, which permits coculturing of human astrocytes and brain endothelial cells on the opposite sides of a porous membrane.

Tags

Keywords: Blood-brain Barrier In Vitro Model Contact Model Primary Human Brain Endothelial Cells Immortalized Human Astrocytes Cell Culture Cell-seeding Research Applications
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter