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Immunology and Infection

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JoVE Journal Immunology and Infection
Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes
 

Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes

Article DOI: 10.3791/51043-v • 14:05 min • September 19th, 2013
September 19th, 2013 •
1,2,3, 1,2,3, 4, 5, 5, 1,2,3, 1,2,3
1Unité des Interactions Bactéries Cellules, Pasteur Institute, 2INSERM U604, 3Institut National de la Recherche Agronomique (INRA), USC2020, 4Institute of Biochemistry, ETH Zürich, 5Focal Area Infection Biology, Biozentrum, University of Basel

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Listeria monocytogenes is a Gram positive bacterial pathogen frequently used as a major model for the study of intracellular parasitism. Imaging late L. monocytogenes infection stages within the context of small-interfering RNA screens allows for the global study of cellular pathways required for bacterial infection of target host cells.

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Keywords: Listeria Monocytogenes Bacterial Pathogen Intracellular Parasitism Cellular Immune Response InlC Fluorescent Labeling Microscopy High-throughput SiRNA Screening Cellular Signaling Pathways
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